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Although simple to perform, these binding assays pose the difficulty of correlating unequivocally the binding sites concerned with the pharmacological effect; this is particularly difficult when more than one population of binding sites is present.
A membrane transporter actively accumulates iodide in thyroid cells; this is followed by oxidation to iodine, iodination of tyrosine residues in thyroglobulin, conjugation of two diiodotyrosine groups, and formation of T4 and T3 moieties. These reactions are catalyzed by thyroid peroxidase, which is localized in the apical border of the follicular cell membrane. |
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